1475 Single cell RNA-seq reveals cell-type specific circadian regulations in the mouse dermis

The molecular circadian clock, which consists of transcription-translation feedback loops, is robust in all skin layers. As dysfunction associated with diseases, understanding the clock may provide insights into disease development and management. Particularly, core machinery interacts elements wound healing process such as fibroblast migration, manipulation network or alternation wounding time changes duration healing. To explore functions dermis, we performed single-cell RNA sequencing on dorsal dermis collected from wild-type C57BL/6J mice every four hours for 72 under regular 12:12 light-dark cycle. Dermal fibroblasts various types dermal immune cells were captured. Consistent previous studies, found that robustly rhythmic dermis. Interestingly, there are more genes (n=1867) than (n = 353), while oscillatory patterns gene expressions similar, amplitude dampened compared to fibroblasts. Circadian prediction outcome a computational pipeline built part this study suggests greater heterogeneity phase causes rhythms. Additionally, some terms related basic cellular function diurnally regulated both cells, have enrichment metabolism terms, whereas responses, particular antivirus responses. These differences not caused by absence expression one cell type, expressed but only one. In summary, highlights differential regulations mouse cells.